Anti-Human CD20 Recombinant Antibody (Rituximab) (CAT#: TAB-016)
Recombinant monoclonal antibody to Human CD20. Rituximab (trade names rituximab and rituximab) is a chimeric monoclonal antibody against the protein CD20, which is primarily found on the surface of immune system B cells (this should not be confused with pancreatic β- or beta cells). Rituximab destroys B cells and is therefore used to treat diseases which are characterized by excessive numbers of B cells, overactive B cells, or dysfunctional B cells. This includes many lymphomas, leukemias, transplant rejection, and autoimmune disorders.
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WB
Figure 1 Anti-CD20 Antibody (TAB-016) in WB
Western blot analysis of TAB-016 was performed with CD20 Protein, Human, Recombinant (TrxA Tag).
TAB-016 incubation concentration: 2ng/μL.
The secondary antibody: HRP-Anti-Human IgG (H+L)
Lane 1: Reducing Antigen (0.1μg)
Lane 2: Reducing Antigen (0.3μg)
Lane 3: Reducing Antigen (0.6μg)
FC
Figure 2 Anti-CD20 Antibody (TAB-016) in Flow Cytometry
Flow cytometry analysis of Daudi cells with Anti-CD20 Antibody (Cat#TAB-016, Blue line) or isotype control antibody (Yellow line). A Goat Anti-human IgG (FITC) was used as the secondary antibody.
ELISA
Figure 3 Anti-Human CD20 Recombinant Antibody (TAB-016) in ELISA
ELISA analysis of TAB-016 was performed by coating with Human CD20, his-Avi tag.
The secondary antibody: Goat Anti-Human IgG-HRP
Specifications
- Immunogen
- Human lymphoblastoid cell line SB
- Host Species
- Mouse
- Derivation
- Chimeric (mouse/human)
- Type
- IgG1 - kappa
- Specificity
- Tested positive against native human antigen
- Species Reactivity
- Human
- Applications
- Suitable for use in Neut, ELISA, IF, IP, FuncS, FC, WB and most other immunological methods.
- Trade name
- rituximab / rituximab
- CAS
- 174722-31-7
- Generic Name
- Rituximab
- Biological Half-Life
- 30 to 400 hours (varies by dose and length of treatment)
- ATC Code
- L01XC02
- DrugBank
- DB00073
- UNII
- 4F4X42SYQ6
- ChEMBL
- CHEMBL1201576
- MW
- 143,859.7 g/mol
- Related Disease
- Wegener's Granulomatosis (WG) and Microscopic Polygamiitis (MPA), in combination with glucocorticoides
Product Property
- Purity
- >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
- Storage
- Store it under sterile conditions at -20°C upon receiving. Recommend to pack the protein into smaller quantities for optimal storage.
Target
- Alternative Names
- Rituximab;rituximab / rituximab;174722-31-7;rituximab;rituximab;rituximab;Zytux;DB00073MS4A1;membrane-spanning 4-domains, subfamily A, member 1;CD20;B-lymphocyte antigen CD20;B1;Bp35;CD20 antigen;CD20 receptor;leukocyte surface antigen Leu-16;B-lymphocyte c
- Gene ID
- 931
- UniProt ID
- P11836
Related Resources
Limongi, Tania, et al. "Extracellular vesicles tropism: A comparative study between passive innate tropism and the active engineered targeting capability of lymphocyte-derived evs." Membranes 11.11 (2021): 886. https://doi.org/10.3390/membranes11110886
This study investigates the targeting capabilities of extracellular vesicles (EVs) derived from B lymphocytes. The researchers conducted a thorough immuno-phenotypic characterization of B-cell EV membranes and explored the tropism of these vesicles towards different cell lines. The study demonstrated that native EVs have an innate capability for passive targeting, which could be significantly enhanced by engineering the vesicles with anti-CD20 monoclonal antibodies. The engineered EVs showed increased internalization into target cancer cells, specifically Daudi cells, which highly express the CD20 marker, compared to HL60 cells that lack this marker.
Creative Biolabs contributed significantly to this research by providing the anti-CD20 monoclonal antibody Rituximab (Cat#: TAB-016). This antibody was crucial for engineering the EVs to enhance their targeting capability towards CD20-expressing cancer cells. The use of this antibody allowed the researchers to demonstrate the increased efficacy of the engineered EVs in selectively targeting and internalizing into the Daudi cell line, thereby validating the potential of antibody-engineered EVs for targeted cancer therapy.
More Infomation
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "Rituximab"
Afuco™ Anti-MS4A1 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-016)This product is an ADCC enhanced antibody produced by our Afuco™ platform. Recombinant monoclonal antibody to Human CD20. It is a chimeric monoclonal antibody against the protein CD20, which is primarily found on the surface of immune system B cells (this should not be confused with pancreatic β- or beta cells).
See other products for "MS4A1"
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Flow Cytometry (FC) related Reagents and Kits: Empowering Cell Function Research
Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-772 | Human Anti-MS4A1 Recombinant Antibody (TAB-772) | ELISA, IP, FC, FuncS, Neut, IF, ICC | IgG1 |
| TAB-771 | Humanized Anti-MS4A1 Recombinant Antibody (TAB-771) | IF, IP, Neut, FuncS, ELISA, FC, ICC | Humanized IgG1, κ |
| TAB-1635CL-S(P) | Anti-Human MS4A1 Recombinant Antibody scFv Fragment (BVX20) | Inhib | Humanized antibody |
| TAB-1636CL-S(P) | Human Anti-MS4A1 Recombinant Antibody; scFv Fragment (TAB-1636CL-S(P)) | ADCC, CDC | Humanized scFv |
| TAB-1637CL-S(P) | Human Anti-MS4A1 Recombinant Antibody; scFv Fragment (TAB-1637CL-S(P)) | ADCC, CDC | Humanized scFv |
Customer Reviews and Q&As
Customer Review
Q&As
Emily Johnson
Excellent for CD20 Research
Excellent for CD20 Research
The Anti-Human CD20 Therapeutic Antibody (rituximab) has significantly improved our CD20 cell studies. The specificity and sensitivity are outstanding, providing clear and reproducible results. This antibody has become essential for our research, enhancing the accuracy and reliability of our assays.
21.Sep,23
David Brown
Effective in Flow Cytometry
Effective in Flow Cytometry
We incorporated this antibody into our flow cytometry experiments, and it's been a great asset. The specificity for CD20 is precise, allowing for accurate cell population analysis. The reliable performance has made it an invaluable tool in our lab.
14.Nov,22
John Wilson
Consistent and Reliable
Consistent and Reliable
This antibody has proven to be consistent and reliable in our immunoassays. The high-quality, reproducible results have significantly improved our experiments, making it a crucial part of our research toolkit. The strong affinity and specificity for CD20 have been particularly beneficial.
19.Jun,21
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# TAB-016, RRID: AB_3111749)
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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